[FULL] Primer 6 And Permanova User Manual: The Ultimate Resource for Multivariate Ecological Researc

The 16S rDNA was amplified in triplicate and barcoded using a previously published protocol (26). Briefly, 1 μL of the extracted DNA was amplified using primers complementary to the V4 (515F/806R) region of the 16S rRNA gene in a single-step PCR reaction. Illumina (San Diego, CA, USA) flow cell adapter sequences and a 12-bp barcode were incorporated into the PCR primers, yielding a fully Illumina-compatible sequencing library. DNA amplicon purity and concentration was quantified on a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, California, USA) and Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).

[FULL] Primer 6 And Permanova User Manuall

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